Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model

Saara Tegelberg†,
Nikica Tomašić†,
Jukka Kallijärvi,
Janne Purhonen,
Eskil Elmér,
Eva Lindberg,
David Gisselsson Nord,
Maria Soller,
Nicole Lesko,
Anna Wedell,
Helene Bruhn,
Christoph Freyer,
Henrik Stranneheim,
Rolf Wibom,
Inger Nennesmo,
Anna Wredenberg,
Erik A. Eklund†Email author and
Vineta Fellman†

†Contributed equally

Orphanet Journal of Rare Diseases201712:73

DOI: 10.1186/s13023-017-0624-2

Received: 23 December 2016

Accepted: 4 April 2017

Published: 20 April 2017

Abstract

Background

Mitochondrial diseases due to defective respiratory chain complex III (CIII) are relatively uncommon. The assembly of the eleven-subunit CIII is completed by the insertion of the Rieske iron-sulfur protein, a process for which BCS1L protein is indispensable. Mutations in the BCS1L gene constitute the most common diagnosed cause of CIII deficiency, and the phenotypic spectrum arising from mutations in this gene is wide.

Results

A case of CIII deficiency was investigated in depth to assess respiratory chain function and assembly, and brain, skeletal muscle and liver histology. Exome sequencing was performed to search for the causative mutation(s). The patient’s platelets and muscle mitochondria showed respiration defects and defective assembly of CIII was detected in fibroblast mitochondria. The patient was compound heterozygous for two novel mutations in BCS1L, c.306A > T and c.399delA. In the cerebral cortex a specific pattern of astrogliosis and widespread loss of microglia was observed. Further analysis showed loss of Kupffer cells in the liver. These changes were not found in infants suffering from GRACILE syndrome, the most severe BCS1L-related disorder causing early postnatal mortality, but were partially corroborated in a knock-in mouse model of BCS1L deficiency.

Conclusions

We describe two novel compound heterozygous mutations in BCS1L causing CIII deficiency. The pathogenicity of one of the mutations was unexpected and points to the importance of combining next generation sequencing with a biochemical approach when investigating these patients. We further show novel manifestations in brain, skeletal muscle and liver, including abnormality in specialized resident macrophages (microglia and Kupffer cells). These novel phenotypes forward our understanding of CIII deficiencies caused by BCS1L mutations.

Keywords

Mitochondrial disorder Respiratory chain Respirometry Assembly factors Blue native gel electrophoresis Encephalopathy Hepatopathy Microglia Barrel cortex